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goat anti ccl2 capture antibody  (R&D Systems)


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    Structured Review

    R&D Systems goat anti ccl2 capture antibody
    Quantitative PCR primer and probe sequences.
    Goat Anti Ccl2 Capture Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+ccl2+capture+antibody/Canine+CCL2%2FJE%2FMCP-1+Antibody/pmc03698075-164-16-21
    Average 90 stars, based on 2 article reviews
    goat anti ccl2 capture antibody - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "A Systematic Analysis of the Peripheral and CNS Effects of Systemic LPS, IL-1Β, TNF-α and IL-6 Challenges in C57BL/6 Mice"

    Article Title: A Systematic Analysis of the Peripheral and CNS Effects of Systemic LPS, IL-1Β, TNF-α and IL-6 Challenges in C57BL/6 Mice

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0069123

    Quantitative PCR primer and probe sequences.
    Figure Legend Snippet: Quantitative PCR primer and probe sequences.

    Techniques Used: Real-time Polymerase Chain Reaction, Sequencing, Amplification

    Liver transcription of mRNA species for IL-1β, TNF-α, CXCL1, CCL2, SAA2 and TNF-αIP2 were measured using quantitative PCR in C57BL/6 female mice 2 hours after systemic challenge (i.p.) with saline, LPS (100 µg/kg), IL-1β (15 µg/kg or 50 µg/kg), TNF-α (50 µg/kg or 250 µg/kg) and IL-6 (50 µg/kg or 125 µg/kg). Data were analysed by one-way ANOVA, followed by Bonferroni post-hoc tests comparing differences between saline, LPS and each cytokine treatment at the higher dose. ##, denotes treatment is significantly different to saline treatment # # p<0.01, # # # p<0.001. * denotes significant difference between treatment groups indicated by line p < 0.05, **p<0.01, ***p<0.001. +++ denotes significant difference between low and high dose of cytokine p<0.001. All data have been presented as mean ± SEM, n=5 for all groups.
    Figure Legend Snippet: Liver transcription of mRNA species for IL-1β, TNF-α, CXCL1, CCL2, SAA2 and TNF-αIP2 were measured using quantitative PCR in C57BL/6 female mice 2 hours after systemic challenge (i.p.) with saline, LPS (100 µg/kg), IL-1β (15 µg/kg or 50 µg/kg), TNF-α (50 µg/kg or 250 µg/kg) and IL-6 (50 µg/kg or 125 µg/kg). Data were analysed by one-way ANOVA, followed by Bonferroni post-hoc tests comparing differences between saline, LPS and each cytokine treatment at the higher dose. ##, denotes treatment is significantly different to saline treatment # # p<0.01, # # # p<0.001. * denotes significant difference between treatment groups indicated by line p < 0.05, **p<0.01, ***p<0.001. +++ denotes significant difference between low and high dose of cytokine p<0.001. All data have been presented as mean ± SEM, n=5 for all groups.

    Techniques Used: Real-time Polymerase Chain Reaction, Saline

    Hippocampal mRNA transcription of CCL2, CXCL1, PTX3 and uPAR was measured using quantitative PCR after systemic challenge (i.p.) with saline, IL-1β (15 µg/kg) or TNF-α at 1, 2, 4, 8 and 24 hours post-injection. All data groups were compared by two-way ANOVA, followed by Bonferroni post-hoc tests comparing differences between each IL-1β or TNF-α group and the equivalent saline groups and comparing the cytokines directly. # denotes treatment is significantly different to saline, while * denotes a difference between IL-1β and TNF-α. #/* p < 0.05, # #/** p < 0.01, # # #/*** p<0.001. All data have been presented as mean±SEM, n=5 for all IL-1β/TNF-α groups, except all 2 hour groups (n=4). All saline groups n≥4 except 4 and 24 hours n≥3.
    Figure Legend Snippet: Hippocampal mRNA transcription of CCL2, CXCL1, PTX3 and uPAR was measured using quantitative PCR after systemic challenge (i.p.) with saline, IL-1β (15 µg/kg) or TNF-α at 1, 2, 4, 8 and 24 hours post-injection. All data groups were compared by two-way ANOVA, followed by Bonferroni post-hoc tests comparing differences between each IL-1β or TNF-α group and the equivalent saline groups and comparing the cytokines directly. # denotes treatment is significantly different to saline, while * denotes a difference between IL-1β and TNF-α. #/* p < 0.05, # #/** p < 0.01, # # #/*** p<0.001. All data have been presented as mean±SEM, n=5 for all IL-1β/TNF-α groups, except all 2 hour groups (n=4). All saline groups n≥4 except 4 and 24 hours n≥3.

    Techniques Used: Real-time Polymerase Chain Reaction, Saline, Injection

    Representative micrographs of vascular NFκB p65, COX-2, CCL2 and CXCL1 2h after i.p. challenge with either saline, LPS (100µg/kg), IL-1β (15µg/kg) or TNF-α (50µg/kg). All pictures were taken from the hippocampus, except for COX-2, which were taken from the thalamus since neuropil labelling with COX-2 is also high in the hippocampus, thus reducing the contrast with activated endothelium. Scale bars are 20µm and 50µm as shown.
    Figure Legend Snippet: Representative micrographs of vascular NFκB p65, COX-2, CCL2 and CXCL1 2h after i.p. challenge with either saline, LPS (100µg/kg), IL-1β (15µg/kg) or TNF-α (50µg/kg). All pictures were taken from the hippocampus, except for COX-2, which were taken from the thalamus since neuropil labelling with COX-2 is also high in the hippocampus, thus reducing the contrast with activated endothelium. Scale bars are 20µm and 50µm as shown.

    Techniques Used: Saline

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: A Systematic Analysis of the Peripheral and CNS Effects of Systemic LPS, IL-1Β, TNF-α and IL-6 Challenges in C57BL/6 Mice
    Article Snippet: .. Primary antibodies were used as follows: mouse anti-p65 (1/1000, Santa Cruz), goat anti-COX2 (1/1000, Santa Cruz), goat anti-CCL2 capture antibody (1/1000, R&D Systems CCL2 duo-set ELISA antibody pair), goat anti-CXCL1 (1/50, R&D Systems), mouse anti-cFOS (1/250, Santa Cruz), rabbit anti-IL-1β (1/50 in 20% normal goat serum, Peprotech) and goat anti-TNF-α capture antibody (1/1000, R&D Systems ELISA kit). .. They were incubated overnight at 4 o C. Sections were then incubated in appropriate biotinylated secondary antibody at 1/100 (Vector Labs) and developed using the ABC method with hydrogen peroxide and diaminobenzidine (DAB) as substrate.



    Similar Products

    goat anti ccl2 capture antibody  (R&D Systems)
    90
    R&D Systems goat anti ccl2 capture antibody
    Quantitative PCR primer and probe sequences.
    Goat Anti Ccl2 Capture Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+ccl2+capture+antibody/Canine+CCL2%2FJE%2FMCP-1+Antibody/pmc03698075-164-16-21
    Average 90 stars, based on 1 article reviews
    goat anti ccl2 capture antibody - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Quantitative PCR primer and probe sequences.

    Journal: PLoS ONE

    Article Title: A Systematic Analysis of the Peripheral and CNS Effects of Systemic LPS, IL-1Β, TNF-α and IL-6 Challenges in C57BL/6 Mice

    doi: 10.1371/journal.pone.0069123

    Figure Lengend Snippet: Quantitative PCR primer and probe sequences.

    Article Snippet: Primary antibodies were used as follows: mouse anti-p65 (1/1000, Santa Cruz), goat anti-COX2 (1/1000, Santa Cruz), goat anti-CCL2 capture antibody (1/1000, R&D Systems CCL2 duo-set ELISA antibody pair), goat anti-CXCL1 (1/50, R&D Systems), mouse anti-cFOS (1/250, Santa Cruz), rabbit anti-IL-1β (1/50 in 20% normal goat serum, Peprotech) and goat anti-TNF-α capture antibody (1/1000, R&D Systems ELISA kit).

    Techniques: Real-time Polymerase Chain Reaction, Sequencing, Amplification

    Liver transcription of mRNA species for IL-1β, TNF-α, CXCL1, CCL2, SAA2 and TNF-αIP2 were measured using quantitative PCR in C57BL/6 female mice 2 hours after systemic challenge (i.p.) with saline, LPS (100 µg/kg), IL-1β (15 µg/kg or 50 µg/kg), TNF-α (50 µg/kg or 250 µg/kg) and IL-6 (50 µg/kg or 125 µg/kg). Data were analysed by one-way ANOVA, followed by Bonferroni post-hoc tests comparing differences between saline, LPS and each cytokine treatment at the higher dose. ##, denotes treatment is significantly different to saline treatment # # p<0.01, # # # p<0.001. * denotes significant difference between treatment groups indicated by line p < 0.05, **p<0.01, ***p<0.001. +++ denotes significant difference between low and high dose of cytokine p<0.001. All data have been presented as mean ± SEM, n=5 for all groups.

    Journal: PLoS ONE

    Article Title: A Systematic Analysis of the Peripheral and CNS Effects of Systemic LPS, IL-1Β, TNF-α and IL-6 Challenges in C57BL/6 Mice

    doi: 10.1371/journal.pone.0069123

    Figure Lengend Snippet: Liver transcription of mRNA species for IL-1β, TNF-α, CXCL1, CCL2, SAA2 and TNF-αIP2 were measured using quantitative PCR in C57BL/6 female mice 2 hours after systemic challenge (i.p.) with saline, LPS (100 µg/kg), IL-1β (15 µg/kg or 50 µg/kg), TNF-α (50 µg/kg or 250 µg/kg) and IL-6 (50 µg/kg or 125 µg/kg). Data were analysed by one-way ANOVA, followed by Bonferroni post-hoc tests comparing differences between saline, LPS and each cytokine treatment at the higher dose. ##, denotes treatment is significantly different to saline treatment # # p<0.01, # # # p<0.001. * denotes significant difference between treatment groups indicated by line p < 0.05, **p<0.01, ***p<0.001. +++ denotes significant difference between low and high dose of cytokine p<0.001. All data have been presented as mean ± SEM, n=5 for all groups.

    Article Snippet: Primary antibodies were used as follows: mouse anti-p65 (1/1000, Santa Cruz), goat anti-COX2 (1/1000, Santa Cruz), goat anti-CCL2 capture antibody (1/1000, R&D Systems CCL2 duo-set ELISA antibody pair), goat anti-CXCL1 (1/50, R&D Systems), mouse anti-cFOS (1/250, Santa Cruz), rabbit anti-IL-1β (1/50 in 20% normal goat serum, Peprotech) and goat anti-TNF-α capture antibody (1/1000, R&D Systems ELISA kit).

    Techniques: Real-time Polymerase Chain Reaction, Saline

    Hippocampal mRNA transcription of CCL2, CXCL1, PTX3 and uPAR was measured using quantitative PCR after systemic challenge (i.p.) with saline, IL-1β (15 µg/kg) or TNF-α at 1, 2, 4, 8 and 24 hours post-injection. All data groups were compared by two-way ANOVA, followed by Bonferroni post-hoc tests comparing differences between each IL-1β or TNF-α group and the equivalent saline groups and comparing the cytokines directly. # denotes treatment is significantly different to saline, while * denotes a difference between IL-1β and TNF-α. #/* p < 0.05, # #/** p < 0.01, # # #/*** p<0.001. All data have been presented as mean±SEM, n=5 for all IL-1β/TNF-α groups, except all 2 hour groups (n=4). All saline groups n≥4 except 4 and 24 hours n≥3.

    Journal: PLoS ONE

    Article Title: A Systematic Analysis of the Peripheral and CNS Effects of Systemic LPS, IL-1Β, TNF-α and IL-6 Challenges in C57BL/6 Mice

    doi: 10.1371/journal.pone.0069123

    Figure Lengend Snippet: Hippocampal mRNA transcription of CCL2, CXCL1, PTX3 and uPAR was measured using quantitative PCR after systemic challenge (i.p.) with saline, IL-1β (15 µg/kg) or TNF-α at 1, 2, 4, 8 and 24 hours post-injection. All data groups were compared by two-way ANOVA, followed by Bonferroni post-hoc tests comparing differences between each IL-1β or TNF-α group and the equivalent saline groups and comparing the cytokines directly. # denotes treatment is significantly different to saline, while * denotes a difference between IL-1β and TNF-α. #/* p < 0.05, # #/** p < 0.01, # # #/*** p<0.001. All data have been presented as mean±SEM, n=5 for all IL-1β/TNF-α groups, except all 2 hour groups (n=4). All saline groups n≥4 except 4 and 24 hours n≥3.

    Article Snippet: Primary antibodies were used as follows: mouse anti-p65 (1/1000, Santa Cruz), goat anti-COX2 (1/1000, Santa Cruz), goat anti-CCL2 capture antibody (1/1000, R&D Systems CCL2 duo-set ELISA antibody pair), goat anti-CXCL1 (1/50, R&D Systems), mouse anti-cFOS (1/250, Santa Cruz), rabbit anti-IL-1β (1/50 in 20% normal goat serum, Peprotech) and goat anti-TNF-α capture antibody (1/1000, R&D Systems ELISA kit).

    Techniques: Real-time Polymerase Chain Reaction, Saline, Injection

    Representative micrographs of vascular NFκB p65, COX-2, CCL2 and CXCL1 2h after i.p. challenge with either saline, LPS (100µg/kg), IL-1β (15µg/kg) or TNF-α (50µg/kg). All pictures were taken from the hippocampus, except for COX-2, which were taken from the thalamus since neuropil labelling with COX-2 is also high in the hippocampus, thus reducing the contrast with activated endothelium. Scale bars are 20µm and 50µm as shown.

    Journal: PLoS ONE

    Article Title: A Systematic Analysis of the Peripheral and CNS Effects of Systemic LPS, IL-1Β, TNF-α and IL-6 Challenges in C57BL/6 Mice

    doi: 10.1371/journal.pone.0069123

    Figure Lengend Snippet: Representative micrographs of vascular NFκB p65, COX-2, CCL2 and CXCL1 2h after i.p. challenge with either saline, LPS (100µg/kg), IL-1β (15µg/kg) or TNF-α (50µg/kg). All pictures were taken from the hippocampus, except for COX-2, which were taken from the thalamus since neuropil labelling with COX-2 is also high in the hippocampus, thus reducing the contrast with activated endothelium. Scale bars are 20µm and 50µm as shown.

    Article Snippet: Primary antibodies were used as follows: mouse anti-p65 (1/1000, Santa Cruz), goat anti-COX2 (1/1000, Santa Cruz), goat anti-CCL2 capture antibody (1/1000, R&D Systems CCL2 duo-set ELISA antibody pair), goat anti-CXCL1 (1/50, R&D Systems), mouse anti-cFOS (1/250, Santa Cruz), rabbit anti-IL-1β (1/50 in 20% normal goat serum, Peprotech) and goat anti-TNF-α capture antibody (1/1000, R&D Systems ELISA kit).

    Techniques: Saline